Time course of prion seeding activity in cerebrospinal fluid of scrapie-infected hamsters after intratongue and intracerebral inoculations.

نویسندگان

  • Christina D Orrù
  • Andrew G Hughson
  • Brent Race
  • Gregory J Raymond
  • Byron Caughey
چکیده

To assess prospects for early diagnosis of prion disease based on prion seeding activity in cerebrospinal fluid (CSF), we measured the activity over time in scrapie-infected hamsters by real-time quaking-induced conversion (RT-QuIC). After intracerebral inoculation, activity appeared in CSF within 1 day and plateaued weeks before the onset of clinical signs. However, after intratongue inoculation, activity first appeared in CSF with the onset of clinical signs, well after higher-level accumulation of seeding activity in brain.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 50 4  شماره 

صفحات  -

تاریخ انتشار 2012